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Domain-wise effect of AvrPto on the tomato transcriptome 6 hours post inoculationExperiment description: A P. syringae mutant deleted in all well-expressed effector proteins delivered AvrPto expressed from a gene in trans on a plasmid. AvrPto forms are WT, inactivated in either the CD-loop or C-terminal domains, or with both domains inactivated. 6 hours after vacuum infiltration at OD 600 0.02 with 0.02% silwet and 10 mM MgCl2, the samples were collected in tubes, then flash frozen in liquid nitrogen for subsequent analysis. Author: Jay Worley Contact: Greg Martin ([email protected]), Boyce Thompson Institute, Cornell University |